SNP Effect predictor
Exome sequenceなどで多くのvariantsが見つかるのだけど、それらがタンパク質に与える影響を知りたい時にどうするか? PolyPhenがよく用いられてるっぽいけど、UCSCのMLでEnsemblにもSNP Effect predictorというツールが加わったらしいのを知ったのでメモ代わりにコピペしとく。
Is there a tool or suggested method to determine how a particular SNP effects these residues?
Using the dbSNP track controls or the primary table itself,
Coding - Synonymous and Coding - Non-Synonymous
is an annotated for some of the data in this location:snp130.func
http://genome.ucsc.edu/FAQ/FAQdownloads#download1
http://genome.ucsc.edu/goldenPath/help/hgTablesHelp.htmlYou can also take any transcript (full or coding only),
swap in the variation, and use this tool to do a translation:
http://genome.ucsc.edu/ -> Utilities (left blue navigation bar) -> DNA Duster
http://users.soe.ucsc.edu/~kent/dnaDust/dnadust.html
We have another table/file to suggest:
snp130CodingDbSnp -- it has more detailed functional annotations provided by dbSNP
Another option is the SNP consequence tool in Ensembl.
On an Ensembl Location page:
http://www.ensembl.org/Homo_sapiens/Location/View?r=6:133017695-133161157
click on "Manage your data"
and select the SNP Effect predictor. This unsurprisingly works off
Ensembl Genes. The functionality is also available in the Ensembl API